Article Dans Une Revue Microbial Drug Resistance Année : 2015

Antibiotic Resistance, Virulence, and Genetic Background of Community-Acquired Uropathogenic Escherichia coli from Algeria

Résumé

ABSTRACT The OXA-48 carbapenemase is mainly encoded by ∼62-kb IncL/M plasmids. However, chromosome-mediated genes have been observed in Escherichia coli isolates. In this work, we investigated the genetic environment of OXA-48 in members of the family Enterobacteriaceae ( n = 22) to understand how the OXA-48-encoding gene is transferred into the E. coli chromosome. The OXA-48-encoding gene was located within intact Tn 1999.2 transposons in the ∼62-kb plasmids or within a truncated variant of Tn 1999.2 for the OXA-48-encoding genes located in the chromosomes of E. coli bacteria. The analysis of the Tn 1999.2 genetic environment revealed an inverted orientation of the transposon in five ∼62-kb plasmids (5/14 [35%]) and in all chromosome inserts ( n = 8). The sequencing of pRA35 plasmid showed that this orientation of Tn 1999.2 and the acquisition of an IS 1R insertion sequence generated a 21.9-kb IS 1R -based composite transposon encoding OXA-48 and designated Tn 6237 . The sequencing of a chromosomal insert encoding OXA-48 also revealed this new transposon in the E. coli chromosome. PCR mapping showed the presence of this element in all strains harboring an OXA-48-encoding chromosomal insert. However, different insertion sites of this transposon were observed in the E. coli chromosome. Overall, these findings indicate a plasticity of the OXA-48 genetic environment mediated by IS 1R insertion sequences. The insertion sequences can induce the transfer of the OXA-encoding gene into E. coli chromosomes and thereby promote its persistence and expression at low levels.
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hal-04889490 , version 1 (15-01-2025)

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Merzouk Yahiaoui, Frédéric Robin, Rabah Bakour, Moufida Hamidi, Richard Bonnet, et al.. Antibiotic Resistance, Virulence, and Genetic Background of Community-Acquired Uropathogenic Escherichia coli from Algeria. Microbial Drug Resistance, 2015, 21 (5), pp.516-526. ⟨10.1089/mdr.2015.0045⟩. ⟨hal-04889490⟩
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