Article Dans Une Revue Antimicrobial Agents and Chemotherapy Année : 2011

Noncovalent Complexes of an Inactive Mutant of CTX-M-9 with the Substrate Piperacillin and the Corresponding Product

Résumé

ABSTRACT We determined the crystal structure of an inactive Ser70Gly mutant of CTX-M-9 in complex with the bulky penicillin piperacillin at precovalent and posthydrolytic stages in the catalytic process. The structures obtained at high resolution were compared with the corresponding structures for the small penicillin benzylpenicillin and the bulky cephalosporin cefotaxime. The findings highlight the key role of the configuration of the carbon adjacent to the acylamino group of the side chain of β-lactams in the precovalent recognition of substrates.

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hal-04889461 , version 1 (15-01-2025)

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David Leyssene, Julien Delmas, Frédéric Robin, Antony Cougnoux, Lucie Gibold, et al.. Noncovalent Complexes of an Inactive Mutant of CTX-M-9 with the Substrate Piperacillin and the Corresponding Product. Antimicrobial Agents and Chemotherapy, 2011, 55 (12), pp.5660-5665. ⟨10.1128/AAC.00245-11⟩. ⟨hal-04889461⟩
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