Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov. - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Microorganisms Année : 2023

Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov.

Guillaume Borrel
  • Fonction : Auteur
Khaled Fadhlaoui
Wajdi Ben Hania
  • Fonction : Auteur
Nadia Gaci
  • Fonction : Auteur
Gérard Pehau-Arnaudet
Prem Prashant Chaudhary
Pascal Vandekerckove
  • Fonction : Auteur
Nathalie Ballet
Monique Alric
  • Fonction : Auteur
Paul William O’toole
  • Fonction : Auteur
Marie-Laure Fardeau
  • Fonction : Auteur
Bernard Ollivier
  • Fonction : Auteur

Résumé

The methanogenic strain Mx-05T was isolated from the human fecal microbiome. A phylogenetic analysis based on the 16S rRNA gene and protein marker genes indicated that the strain is affiliated with the order Methanomassiliicoccales. It shares 86.9% 16S rRNA gene sequence identity with Methanomassiliicoccus luminyensis, the only member of this order previously isolated. The cells of Mx-05T were non-motile cocci, with a diameter range of 0.4–0.7 μm. They grew anaerobically and reduced methanol, monomethylamine, dimethylamine, and trimethylamine into methane, using H2 as an electron donor. H2/CO2, formate, ethanol, and acetate were not used as energy sources. The growth of Mx-05T required an unknown medium factor(s) provided by Eggerthella lenta and present in rumen fluid. Mx-05T grew between 30 °C and 40 °C (optimum 37 °C), over a pH range of 6.9–8.3 (optimum pH 7.5), and between 0.02 and 0.34 mol.L−1 NaCl (optimum 0.12 mol.L−1 NaCl). The genome is 1.67 Mbp with a G+C content of 55.5 mol%. Genome sequence annotation confirmed the absence of the methyl branch of the H4MPT Wood–Ljungdahl pathway, as described for other Methanomassiliicoccales members. Based on an average nucleotide identity analysis, we propose strain Mx-05T as being a novel representative of the order Methanomassiliicoccales, within the novel family Methanomethylophilaceae, for which the name Methanomethylophilus alvi gen. nov, sp. nov. is proposed. The type strain is Mx-05T (JCM 31474T).

Dates et versions

hal-04306127 , version 1 (24-11-2023)

Identifiants

Citer

Guillaume Borrel, Khaled Fadhlaoui, Wajdi Ben Hania, Nadia Gaci, Gérard Pehau-Arnaudet, et al.. Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov.. Microorganisms, 2023, 11 (11), pp.2794. ⟨10.3390/microorganisms11112794⟩. ⟨hal-04306127⟩
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