Synthesis, characterization, crystal structure and HSA binding of two new N,O,O-donor Schiff-base ligands derived from dihydroxybenzaldehyde and tert-butylamine - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Journal of Molecular Structure Année : 2016

Synthesis, characterization, crystal structure and HSA binding of two new N,O,O-donor Schiff-base ligands derived from dihydroxybenzaldehyde and tert-butylamine

Résumé

Two new o-hydroxy Schiff-bases compounds, L 1 and L 2 , were derived from the 1:1 M condensation of 2,3-dihydroxybenzaldehyde and 2,4-dihydroxybenzaldehyde with tertbutylamine and were characterized by elemental analysis, FT-IR, 1 H and 13 C NMR spectroscopies. The crystal structure of L 2 was also determined by single crystal X-ray analysis. The crystal structure of L 2 showed that the compound exists as a zwitterionic form in the solid state, with the H atom of the phenol group being transferred to the imine N atom. It adopts an E configuration about the central C N double bond. Furthermore, binding of these Schiff base ligands to Human Serum Albumin (HSA) was investigated by fluorescence quenching, absorption spectroscopy, molecular docking and molecular dynamics (MD) simulation methods. The fluorescence emission of HSA was quenched by ligands. Also, suitable models were used to analyze the UV-vis absorption spectroscopy data for titration of HSA solution by various amounts of Schiff bases. The spectroscopic studies revealed that these Schiff bases formed 1:1 complex with HSA. Energy transfer mechanism of quenching was discussed and the values of 3.35 and 1.57 nm as the mean distances between the bound ligands and the HSA were calculated for L 1 and L 2 , respectively. Molecular docking results indicated that the main active binding site for these Schiff bases ligands is in subdomain IB. Moreover, MD simulation results suggested that this Schiff base complex can interact with HSA, with a slight modification of its tertiary structure.
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Dates et versions

hal-04087564 , version 1 (03-05-2023)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Iman Khosravi, Farnaz Hosseini, Mahsa Khorshidifard, Mehdi Sahihi, Hadi Amiri Rudbari. Synthesis, characterization, crystal structure and HSA binding of two new N,O,O-donor Schiff-base ligands derived from dihydroxybenzaldehyde and tert-butylamine. Journal of Molecular Structure, 2016, 1119, pp.373-384. ⟨10.1016/j.molstruc.2016.04.094⟩. ⟨hal-04087564⟩

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