Carbohydrate Moieties of Microsporidian Polar Tube Proteins Are Targeted by Immunoglobulin G in Immunocompetent Individuals - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Infection and Immunity Année : 2005

Carbohydrate Moieties of Microsporidian Polar Tube Proteins Are Targeted by Immunoglobulin G in Immunocompetent Individuals

Ron Peek
  • Fonction : Auteur
Frédéric Delbac
  • Fonction : Auteur
Dave Speijer
  • Fonction : Auteur
Valérie Polonais
Sophie Greve
  • Fonction : Auteur
Ellen Wentink-Bonnema
  • Fonction : Auteur
Jeffrey Ringrose
  • Fonction : Auteur
Tom van Gool
  • Fonction : Auteur

Résumé

ABSTRACT Microsporidia of the Encephalitozoon species are frequently found as opportunistic pathogens of immunocompromised patients, but very little is known about the prevalence and significance of Encephalitozoon infection in immunocompetent individuals. It was reported previously that 8% of Dutch blood donors and 5% of pregnant French women had an immunoglobulin G (IgG) immune response against specific organelles of Encephalitozoon intestinalis. These organelles, the so-called polar tube and anchoring disk, are used to penetrate membranes of host cells during infection. The unexpectedly high percentage of immunocompetent individuals with IgG against these organelles suggested that infection of humans with microsporidia might be more common than previously recognized. In the present study, we analyzed this anti- Encephalitozoon IgG response by using indirect immunofluorescence, Western blotting, two-dimensional gel electrophoresis, and chemical deglycosylation. Our results show that the antibody response is directed against the posttranslational carbohydrate modification of the major polar tube protein (polar tube protein 1) and carbohydrate moieties of proteins in the anchoring region of the polar tube of Encephalitozoon . In addition, the antibodies were found to decrease the infectivity of E. intestinalis in vitro. The significance and possible origin of these prevalent antibodies are discussed.

Dates et versions

hal-03844656 , version 1 (08-11-2022)

Identifiants

Citer

Ron Peek, Frédéric Delbac, Dave Speijer, Valérie Polonais, Sophie Greve, et al.. Carbohydrate Moieties of Microsporidian Polar Tube Proteins Are Targeted by Immunoglobulin G in Immunocompetent Individuals. Infection and Immunity, 2005, 73 (12), pp.7906-7913. ⟨10.1128/IAI.73.12.7906-7913.2005⟩. ⟨hal-03844656⟩

Collections

PRES_CLERMONT
11 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More