Prmt5 promotes vascular morphogenesis independently of its methyltransferase activity - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue PLoS Genetics Année : 2021

Prmt5 promotes vascular morphogenesis independently of its methyltransferase activity

Résumé

During development, the vertebrate vasculature undergoes major growth and remodeling. While the transcriptional cascade underlying blood vessel formation starts to be better characterized, little is known concerning the role and mode of action of epigenetic enzymes during this process. Here, we explored the role of the Protein Arginine Methyl Transferase Prmt5 in blood vessel formation as well as hematopoiesis using zebrafish as a model system. Through the combination of different prmt5 loss-of-function approaches we highlighted a key role of Prmt5 in both processes. Notably, we showed that Prmt5 promotes vascular morphogenesis through the transcriptional control of ETS transcription factors and adhesion proteins in endothelial cells. Interestingly, using a catalytic dead mutant of Prmt5 and a specific drug inhibitor, we found that while Prmt5 methyltransferase activity was required for blood cell formation, it was dispensable for vessel formation. Analyses of chromatin architecture impact on reporter genes expression and chromatin immunoprecipitation experiments led us to propose that Prmt5 regulates transcription by acting as a scaffold protein that facilitates chromatin looping to promote vascular morphogenesis.
Fichier principal
Vignette du fichier
journal.pgen.1009641-4.pdf (3.74 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03430618 , version 1 (02-12-2020)
hal-03430618 , version 2 (16-11-2021)

Licence

Paternité

Identifiants

Citer

Aurélie Quillien, Guerric Gilbert, Manon Boulet, Séverine Ethuin, Lucas Waltzer, et al.. Prmt5 promotes vascular morphogenesis independently of its methyltransferase activity. PLoS Genetics, 2021, 17 (6), pp.e1009641. ⟨10.1371/journal.pgen.1009641⟩. ⟨hal-03430618v2⟩
87 Consultations
43 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More