Continental arc lavas display geochemical signatures that reflect both mantle metasomatism by slab fluids or melts and extensive differentiation of magmas within crustal reservoirs. The relative effect of source and crustal processes are difficult to disentangle based on whole-rock compositions. This issue is critical in Ecuador where volcanism occurs through a thick continental crust (>50 km). This study reconstructs the history of melts feeding the Guagua Pichincha volcano, Western Cordillera, by analysing the Pb isotope composition and major-trace element content of individual minerals (33 amphiboles, 4 orthopyroxenes and 18 plagioclases) hosted in two dacite samples. It uses a low-blank wet-chemistry method for precise analysis of Pb amounts as low as 150 pg.Early crystallized, high-Al amphiboles with Al2O3 ≥ 9.8 wt% and Eu/Eu* > 0.7 have the lowest and most heterogeneous 206Pb/204Pb (18.816–18.999), whereas plagioclases have the highest and most homogeneous 206Pb/204Pb (19.003–19.023). Low-Al amphiboles and orthopyroxenes display intermediate compositions and variability (18.934–19.007). The 206Pb/204Pb ratio correlates negatively with Eu/Eu* in amphiboles and orthopyroxenes, which indicates that the Guagua Pichincha magmas assimilate radiogenic Pb within the stability field of plagioclase (i.e. in the upper crust). The radiogenic ankaramites of the Guaranda unit, an accreted ocean terrain making the basement of the Western Cordillera, are the most suitable contaminant. If this is correct, the 206Pb/204Pb increase from the two most primitive amphiboles to their respective host rocks requires ca. 20% crustal assimilation, which is higher than previous estimates in the Northern Volcanic Zone but similar to those inferred for Central Andean mafic lavas. The two most primitive amphiboles with no significant Eu anomaly record the composition of melts before plagioclase crystallization. These deep melts have contrasted 206Pb/204Pb ratios (18.816–18.879) and contents of fluid mobile elements (Li, Cu, Rb, Pb) that probably reflect the input of different slab components to the mantle wedge. Melts in equilibrium with the two most primitive amphiboles of the Guagua Pichincha volcano are enriched in incompatible elements, but depleted in fluid mobile elements compared to the olivine-hosted melt inclusions of the older Rucu Pichincha volcano. This supports previous inferences based on whole-rock data that the mantle source of the Pichincha Volcanic Complex has changed through time.