<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04427983</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T08:44:47+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae</title>
            <author role="aut">
              <persName>
                <forename type="first">Maurine</forename>
                <surname>Fayet</surname>
              </persName>
              <idno type="halauthorid">2527270-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nastasia</forename>
                <surname>Prybylski</surname>
              </persName>
              <idno type="halauthorid">1276273-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie-Laure</forename>
                <surname>Collin</surname>
              </persName>
              <idno type="halauthorid">859411-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Eric</forename>
                <surname>Peyretaillade</surname>
              </persName>
              <email type="md5">8ead48186689bf3b2bf1443660c7ec3a</email>
              <email type="domain">iut.u-clermont1.fr</email>
              <idno type="idhal" notation="numeric">1246149</idno>
              <idno type="halauthorid" notation="string">445747-1246149</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=pE-bVIsAAAAJ</idno>
              <idno type="IDREF">https://www.idref.fr/153387092</idno>
              <idno type="VIAF">https://viaf.org/viaf/215331963</idno>
              <idno type="ISNI">http://isni.org/isni/0000000359044086</idno>
              <idno type="ORCID">https://orcid.org/0009-0009-0140-2136</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Ivan</forename>
                <surname>Wawrzyniak</surname>
              </persName>
              <email type="md5">fd157200786fda81cb15b9cfe9d4022c</email>
              <email type="domain">uca.fr</email>
              <idno type="idhal" notation="string">ivan-wawrzyniak</idno>
              <idno type="idhal" notation="numeric">763269</idno>
              <idno type="halauthorid" notation="string">498526-763269</idno>
              <idno type="IDREF">https://www.idref.fr/16450558X</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5362-6117</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=KoTlR0MAAAAJ</idno>
              <idno type="VIAF">https://viaf.org/viaf/311495636</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Abdel</forename>
                <surname>Belkorchia</surname>
              </persName>
              <idno type="halauthorid">499904-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Reginald Florian</forename>
                <surname>Akossi</surname>
              </persName>
              <idno type="halauthorid">2943471-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie</forename>
                <surname>Diogon</surname>
              </persName>
              <idno type="halauthorid">244096-0</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Hicham</forename>
                <surname>El Alaoui</surname>
              </persName>
              <email type="md5">2e34fac5d5f1e71662948a155b488d18</email>
              <email type="domain">univ-bpclermont.fr</email>
              <idno type="idhal" notation="numeric">1058077</idno>
              <idno type="halauthorid" notation="string">497619-1058077</idno>
              <idno type="IDREF">https://www.idref.fr/164593705</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-1926-0905</idno>
              <idno type="VIAF">https://viaf.org/viaf/313552990</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Valérie</forename>
                <forename type="middle">VP</forename>
                <surname>Polonais</surname>
              </persName>
              <email type="md5">dd57bcdcf74b88f7300b6663458daa35</email>
              <email type="domain">uca.fr</email>
              <idno type="idhal" notation="string">valerie-polonais</idno>
              <idno type="idhal" notation="numeric">179753</idno>
              <idno type="halauthorid" notation="string">3036400-179753</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2455-4425</idno>
              <idno type="IDREF">https://www.idref.fr/110540670</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Frédéric</forename>
                <forename type="middle">FD</forename>
                <surname>Delbac</surname>
              </persName>
              <email type="md5">19d3a4640b39d992b4900fdb95f4b987</email>
              <email type="domain">univ-bpclermont.fr</email>
              <idno type="idhal" notation="numeric">1315271</idno>
              <idno type="halauthorid" notation="string">3036401-1315271</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-1842-0276</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=WiQYKgsAAAAJ</idno>
              <idno type="IDREF">https://www.idref.fr/153815000</idno>
              <idno type="VIAF">https://viaf.org/viaf/289837275</idno>
              <idno type="ISNI">http://isni.org/isni/0000000395189960</idno>
              <affiliation ref="#struct-1063680"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Valérie</forename>
                <surname>Polonais</surname>
              </persName>
              <email type="md5">dd57bcdcf74b88f7300b6663458daa35</email>
              <email type="domain">uca.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2024-02-13 08:48:35</date>
              <date type="whenModified">2024-09-04 11:06:16</date>
              <date type="whenReleased">2024-02-13 09:09:57</date>
              <date type="whenProduced">2023-05-30</date>
              <date type="whenEndEmbargoed">2024-02-13</date>
              <ref type="file" target="https://uca.hal.science/hal-04427983v1/document">
                <date notBefore="2024-02-13"/>
              </ref>
              <ref type="file" subtype="publisherPaid" n="1" target="https://uca.hal.science/hal-04427983v1/file/s41598-023-35511-y.pdf" id="file-4454204-3866269">
                <date notBefore="2024-02-13"/>
              </ref>
              <ref type="externalLink" target="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229552"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="493527">
                <persName>
                  <forename>Valérie</forename>
                  <surname>Polonais</surname>
                </persName>
                <email type="md5">dd57bcdcf74b88f7300b6663458daa35</email>
                <email type="domain">uca.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04427983</idno>
            <idno type="halUri">https://uca.hal.science/hal-04427983</idno>
            <idno type="halBibtex">fayet:hal-04427983</idno>
            <idno type="halRefHtml">&lt;i&gt;Scientific Reports&lt;/i&gt;, 2023, 13, pp.8773. &lt;a target="_blank" href="https://dx.doi.org/10.1038/s41598-023-35511-y"&gt;&amp;#x27E8;10.1038/s41598-023-35511-y&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Scientific Reports, 2023, 13, pp.8773. &amp;#x27E8;10.1038/s41598-023-35511-y&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0 - Attribution<ref corresp="#file-4454204-3866269"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="PRES_CLERMONT">Université de Clermont</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="LMGE" corresp="PRES_CLERMONT">Laboratoire Microorganismes : Génome et Environnement</idno>
            <idno type="stamp" n="ACL-SVSAE" corresp="PRES_CLERMONT">ACL en SVSAE</idno>
            <idno type="stamp" n="TEST4-HALCNRS">collection test</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Maurine</forename>
                    <surname>Fayet</surname>
                  </persName>
                  <idno type="halauthorid">2527270-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nastasia</forename>
                    <surname>Prybylski</surname>
                  </persName>
                  <idno type="halauthorid">1276273-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie-Laure</forename>
                    <surname>Collin</surname>
                  </persName>
                  <idno type="halauthorid">859411-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Eric</forename>
                    <surname>Peyretaillade</surname>
                  </persName>
                  <email type="md5">8ead48186689bf3b2bf1443660c7ec3a</email>
                  <email type="domain">iut.u-clermont1.fr</email>
                  <idno type="idhal" notation="numeric">1246149</idno>
                  <idno type="halauthorid" notation="string">445747-1246149</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=pE-bVIsAAAAJ</idno>
                  <idno type="IDREF">https://www.idref.fr/153387092</idno>
                  <idno type="VIAF">https://viaf.org/viaf/215331963</idno>
                  <idno type="ISNI">http://isni.org/isni/0000000359044086</idno>
                  <idno type="ORCID">https://orcid.org/0009-0009-0140-2136</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Ivan</forename>
                    <surname>Wawrzyniak</surname>
                  </persName>
                  <email type="md5">fd157200786fda81cb15b9cfe9d4022c</email>
                  <email type="domain">uca.fr</email>
                  <idno type="idhal" notation="string">ivan-wawrzyniak</idno>
                  <idno type="idhal" notation="numeric">763269</idno>
                  <idno type="halauthorid" notation="string">498526-763269</idno>
                  <idno type="IDREF">https://www.idref.fr/16450558X</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5362-6117</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=KoTlR0MAAAAJ</idno>
                  <idno type="VIAF">https://viaf.org/viaf/311495636</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Abdel</forename>
                    <surname>Belkorchia</surname>
                  </persName>
                  <idno type="halauthorid">499904-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Reginald Florian</forename>
                    <surname>Akossi</surname>
                  </persName>
                  <idno type="halauthorid">2943471-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie</forename>
                    <surname>Diogon</surname>
                  </persName>
                  <idno type="halauthorid">244096-0</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Hicham</forename>
                    <surname>El Alaoui</surname>
                  </persName>
                  <email type="md5">2e34fac5d5f1e71662948a155b488d18</email>
                  <email type="domain">univ-bpclermont.fr</email>
                  <idno type="idhal" notation="numeric">1058077</idno>
                  <idno type="halauthorid" notation="string">497619-1058077</idno>
                  <idno type="IDREF">https://www.idref.fr/164593705</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-1926-0905</idno>
                  <idno type="VIAF">https://viaf.org/viaf/313552990</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Valérie</forename>
                    <forename type="middle">VP</forename>
                    <surname>Polonais</surname>
                  </persName>
                  <email type="md5">dd57bcdcf74b88f7300b6663458daa35</email>
                  <email type="domain">uca.fr</email>
                  <idno type="idhal" notation="string">valerie-polonais</idno>
                  <idno type="idhal" notation="numeric">179753</idno>
                  <idno type="halauthorid" notation="string">3036400-179753</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2455-4425</idno>
                  <idno type="IDREF">https://www.idref.fr/110540670</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Frédéric</forename>
                    <forename type="middle">FD</forename>
                    <surname>Delbac</surname>
                  </persName>
                  <email type="md5">19d3a4640b39d992b4900fdb95f4b987</email>
                  <email type="domain">univ-bpclermont.fr</email>
                  <idno type="idhal" notation="numeric">1315271</idno>
                  <idno type="halauthorid" notation="string">3036401-1315271</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-1842-0276</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=WiQYKgsAAAAJ</idno>
                  <idno type="IDREF">https://www.idref.fr/153815000</idno>
                  <idno type="VIAF">https://viaf.org/viaf/289837275</idno>
                  <idno type="ISNI">http://isni.org/isni/0000000395189960</idno>
                  <affiliation ref="#struct-1063680"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">89181</idno>
                <idno type="issn">2045-2322</idno>
                <idno type="eissn">2045-2322</idno>
                <title level="j">Scientific Reports</title>
                <imprint>
                  <publisher>Nature Publishing Group</publisher>
                  <biblScope unit="volume">13</biblScope>
                  <biblScope unit="pp">8773</biblScope>
                  <date type="datePub">2023-05-30</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1038/s41598-023-35511-y</idno>
              <idno type="pubmed">37253964</idno>
              <idno type="pubmedcentral">PMC10229552</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Cytoplasm / metabolism</classCode>
              <classCode scheme="mesh">Fungal Proteins / genetics</classCode>
              <classCode scheme="mesh">Fungal Proteins / metabolism</classCode>
              <classCode scheme="mesh">Microsporidia / genetics</classCode>
              <classCode scheme="mesh">Microsporidia / metabolism</classCode>
              <classCode scheme="mesh">Organelles / metabolism</classCode>
              <classCode scheme="mesh">Animals</classCode>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, which is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsporidian Anncaliia algerae . While PTP1 and PTP2 are found on the whole polar tube, PTP3 is present in a large part of the extruded polar tube except at its end-terminal part. On the contrary, PTP4 is specifically detected at the end-terminal part of the polar tube. To complete PTPs repertoire, sequential sporal protein extractions were done with high concentration of reducing agents. In addition, a method to purify polar tubes was developed. Mass spectrometry analysis conducted on both samples led to the identification of a PTP3-like protein (PTP3b), and a new PTP (PTP7) only found at the extremity of the polar tube. The specific localization of PTPs asks the question of their roles in cell invasion processes used by A. algerae .</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-1063680" status="VALID">
          <idno type="IdRef">150695632</idno>
          <idno type="ISNI">0000000403850000</idno>
          <idno type="RNSR">200012122F</idno>
          <idno type="ROR">https://ror.org/020ahr442</idno>
          <orgName>Laboratoire Microorganismes : Génome et Environnement</orgName>
          <orgName type="acronym">LMGE</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>Campus Universitaire des Cézeaux, TSA 60026, 1 Impasse Amélie Murat, 63178 Aubière</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.lmge.univ-bpclermont.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR6023" active="#struct-441569" type="direct"/>
            <relation name="UMR6023" active="#struct-1063463" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-1063463" status="VALID">
          <idno type="IdRef">252404955</idno>
          <idno type="ISNI">0000000115480420</idno>
          <idno type="ROR">https://ror.org/01a8ajp46</idno>
          <orgName>Université Clermont Auvergne</orgName>
          <orgName type="acronym">UCA</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>49, bd François-Mitterrand / CS 60032 / 63001 Clermont-Ferrand Cedex 1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.uca.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>