Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides - Université Clermont Auvergne Access content directly
Journal Articles Marine drugs Year : 2023

Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides

Meriem Aitouguinane
  • Function : Author
Zainab El Alaoui-Talibi
  • Function : Author
Halima Rchid
Imen Fendri
Slim Abdelkafi
Mohamed Didi Ould El-Hadj
  • Function : Author
Zakaria Boual
Christophe Rihouey
Christine Gardarin
  • Function : Author
Pascal Dubessay
  • Function : Author
Philippe Michaud
Guillaume Pierre
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Cherkaoui El Modafar
  • Function : Author

Abstract

Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (Mw) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants.

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Biotechnology

Dates and versions

hal-04111782 , version 1 (31-05-2023)

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Meriem Aitouguinane, Zainab El Alaoui-Talibi, Halima Rchid, Imen Fendri, Slim Abdelkafi, et al.. Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides. Marine drugs, 2023, 21 (5), pp.301. ⟨10.3390/md21050301⟩. ⟨hal-04111782⟩
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