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Poster De Conférence Année : 2021

Colonic physicochemical parameters from different dog’s sizes reshape canine microbiota activity and structure in an in vitro gut model

Résumé

Canine digestion is a complex and regionalized process involving physicochemical, mechanical, and microbial aspects, playing a key role in maintaining dog health. However, decades of breeding selection are associated to variations in dog’s size, digestive physiology, and disease sensitivity. To reproduce the colonic environmental conditions from three dog’s sizes in vitro, we reviewed the literature to determine canine ileal effluent composition, colonic pH and transit time of small, medium and large dogs. The one stage fermentation system M-ARCOL (Mucosal Artificial Colon) was set-up with those different parameters and bioreactors reproducing the three dog’s size conditions were inoculated with the same fecal sample and ran in parallel for 8 days. Two biological replicates were performed with faecal samples from one male and one female of medium size. Microbiota activity was followed by daily analysis of gas and short-chain fatty acid composition and production. Quantitative PCR (total bacteria) and Illumina 16S sequencing were performed to investigate the structure of luminal and mucus-associated microbiota. Small, medium, and large conditions led to three different profiles. Gas production increased with dog’s size and the same significant positive correlation was observed with total SCFA production, and acetate and butyrate ratios, reflecting the longer fermentation time in large dog colon. qPCR analysis showed a decrease in total bacteria amount from small to large conditions, but influence of dog size on microbiota structure will be confirmed by ongoing Illumina sequencing analysis. To conclude, the sole colonic physicochemical parameters seem to be able to reshape canine microbiota activity and composition. In a next step, bioreactors set with small, medium, and large parameters will be inoculated with corresponding dog faeces to complete the development of in vitro gut models for each dog size and validate them by comparing in vitro results with in vivo data in dogs.
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Dates et versions

hal-03402028 , version 1 (25-10-2021)

Identifiants

  • HAL Id : hal-03402028 , version 1

Citer

Charlotte Deschamps, Humbert Delphine, Nathalie Priymenko, Sylvain Denis, Emmanuelle Apper, et al.. Colonic physicochemical parameters from different dog’s sizes reshape canine microbiota activity and structure in an in vitro gut model. 12. International Symposium on Gut Microbiology, Oct 2021, En ligne, France. 2021. ⟨hal-03402028⟩
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