the aim of this work was to demonstrate the feasibility of second-generation bioethanol production using for the first time a sawmill mixed feedstock comprising four softwood species, representative of biomass resource in auvergne-rhone-alpes region (france). the feedstock was subjected to a microwave-assisted water/ethanol organosolv pretreatment. the investigation focused on enzymatic hydrolysis of this pretreated sawmill feedstock (psf) using cellic® ctec2 as the enzyme, followed by fermentation of the resulting sugar solution using saccharomyces cerevisiae strain. the cellulose-rich psf with 71% w/w cellulose content presented high saccharification yields (up to 80%), which made it perfect for subsequent fermentation; this yield was predicted vs. time up to 5.2% w/v psf loading using a mathematical model fitted only on data at 1.5%. finally, high psf loading (7.5%) and scaleup were shown to impair the saccharification yield, but alcoholic fermentation could still be carried out up to 80% of the theoretical glucose-to-ethanol conversion yield.