Fluid shear stress: a modulator of the vascular protective effects of curcumin? - Université Clermont Auvergne Accéder directement au contenu
Poster De Conférence Année : 2016

Fluid shear stress: a modulator of the vascular protective effects of curcumin?

Dragan Milenkovic
Christine Morand

Résumé

A growing number of evidences suggest that dietary polyphenols exhibit vascular protective properties by preventing the early stages of vascular dysfunction and atherosclerosis development. Using holistic nutrigenomic approaches, we have previously demonstrated that polyphenols may protect the integrity of vascular endothelium by reducing interactions between monocytes and endothelial cells. One important determinant of these inter-cellular interactions is the hemodynamic forces associated with blood flow, namely shear-stress. Usually, in-vitro studies aiming to decipher the impact of polyphenols on the molecular and cellular processes, are performed under static conditions. As chronic exposure of endothelial cells to laminar flow is known to affect endothelial cell functions, the aim of this work is to evaluate the impact of shear stress on the response of endothelial cells to polyphenols exposure. Primary human endothelial cells were grown under a 1Hz-pulsatile aortic shear-stress of 7 dyne/cm² to mimic as closely as possible physiological conditions, or under static conditions. Confluent cells were exposed to low concentrations of curcumin (0.5-1μM) for 3 hours prior to their stimulation by TNF-alpha for 4 hours and then cell adhesion assays were performed. Our first results demonstrate that the pre-exposure of endothelial cells to 0.5µM curcumin under a physiological flow reduced by 40% the adhesion of THP1 monocytes while only a 25% decrease is observed in static condition. This observation suggests that shear-stress can modulate the cellular effects of polyphenols. Further experimentations are ongoing to identify the impact of this shear-stress on the nutrigenomic and molecular responses to curcumin in endothelial cells
Fichier non déposé

Dates et versions

hal-02739352 , version 1 (02-06-2020)

Identifiants

  • HAL Id : hal-02739352 , version 1
  • PRODINRA : 410926

Citer

Laurent-Emmanuel Monfoulet, Dragan Milenkovic, Christine Morand. Fluid shear stress: a modulator of the vascular protective effects of curcumin?. COST POSITIVe Workshop, Mar 2016, Bucarest, Hungary. 2016, COST POSITIVe Workshop. ⟨hal-02739352⟩
4 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More