The p300/CBP-associated factor (PCAF) is a cofactor of ATF4 for amino acid-regulated transcription of CHOP - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2007

The p300/CBP-associated factor (PCAF) is a cofactor of ATF4 for amino acid-regulated transcription of CHOP

Résumé

When an essential amino acid is limited, a signaling cascade is triggered that leads to increased translation of the 'master regulator', activating transcription factor 4 (ATF4), and resulting in the induction of specific target genes. Binding of ATF4 to the amino acid response element (AARE) is an essential step in the transcriptional activation of CHOP (a CCAAT/enhancer-binding protein-related gene) by amino acid deprivation. We set out to identify proteins that interact with ATF4 and that play a role in the transcriptional activation of CHOP. Using a tandem affinity purification (TAP) tag approach, we identified p300/CBP-associated factor (PCAF) as a novel interaction partner of ATF4 in leucine-starved cells. We show that the N-terminal region of ATF4 is required for a direct interaction with PCAF and demonstrate that PCAF is involved in the full transcriptional response of CHOP by amino acid starvation. Chromatin immunoprecipitation analysis revealed that PCAF is engaged on the CHOP AARE in response to amino acid starvation and that ATF4 is essential for its recruitment. We also show that PCAF stimulates ATF4-driven transcription via its histone acetyltransferase domain. Thus PCAF acts as a coactivator of ATF4 and is involved in the enhancement of CHOP transcription following amino acid starvation.

Mots clés

Fichier principal
Vignette du fichier
2007_Chérasse_Nucleic Acids Research_1.pdf (404.66 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02658408 , version 1 (30-05-2020)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Yoan Cherasse, Anne-Catherine Maurin, Cédric Chaveroux, Céline Jousse, Valerie Carraro, et al.. The p300/CBP-associated factor (PCAF) is a cofactor of ATF4 for amino acid-regulated transcription of CHOP. Nucleic Acids Research, 2007, 35 (17), pp.5954-5965. ⟨10.1093/nar/gkm642⟩. ⟨hal-02658408⟩
14 Consultations
44 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More