The aim of this Research Topic on “Sedentary behaviors at work” was to provide a multidisciplinary focus on this area of research. We aimed to give the reader a global overview of sedentary behaviors at work through addressing various aspects. Sedentary behaviors are a leading cause of preventable mortality in developed countries. We mainly have sedentary behaviors at work. Therefore, sedentary behaviors must be considered as an occupational risk, and must be a major concern both for companies/managers and physicians/health researchers. However, sedentary behaviors at work were only studied recently over the past decade and many aspects are still poorly studied. This compilation of several relevant original articles and reviews is the result of the contribution of multidisciplinary researchers from several institutes promoting health and prevention. We would like to thank the authors for sharing their exciting work, showing the wide growing interest on this Research Topic, with eminent specialists ranging from medicine and physiology, to psychology, economics, or engineering. Readers may find this editorial a helpful guide to provide an overview of our Research Topic on “Sedentary behaviors at work.”