GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locations. - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Molecular Reproduction and Development Année : 2002

GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locations.

Résumé

In mice, GPX5 is a secreted protein abundantly synthesized by the caput epididymidis. The protein is secreted as early as the initial segment of the caput and is found subsequently associated with the sperm plasma membrane in a sub-acrosomic localization. We show here that GPX5 is present in the caput and cauda epididymides lumens in three different locations: either free as a soluble protein in the caput epididymal fluid, weakly bound to caput sperm membranes, or, finally, associated to lipid-containing structures conferring to the protein a protective effect against proteolytic digestions. Within the cauda epididymidis, the amount of free GPX5 is low compared to the caput and the association with sperm membranes proved to be more solid. In both caput and cauda sperm samples, the association of GPX5 with the sperm membrane protects GPX5 from proteolytic cleavages. Protection against proteolytic digestions can be overcome by physical treatments of epididymal fluid and sperm samples such as ultrasounds or very acidic pH. These data suggest that complex phenomena and structures participate in the transfer and binding of the caput-secreted GPX5 protein to the sperm plasma membrane.
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Dates et versions

hal-02085768 , version 1 (31-03-2019)

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  • HAL Id : hal-02085768 , version 1

Citer

Rejraji Hanae, Patrick Vernet, Joël Drevet. GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locations.. Molecular Reproduction and Development, 2002, 63 (1), pp.96-103. ⟨hal-02085768⟩

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