<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-01928174</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-03T02:26:18+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Saccharomyces cerevisiae CNCM I-3856 Prevents Colitis Induced by AIEC Bacteria in the Transgenic Mouse Model Mimicking Crohnʼs Disease</title>
            <author role="aut">
              <persName>
                <forename type="first">Adeline</forename>
                <surname>Sivignon</surname>
              </persName>
              <email type="md5">bc8b84f6fbc72565dfdc4885feb27115</email>
              <email type="domain">uca.fr</email>
              <idno type="idhal" notation="string">adeline-sivignon</idno>
              <idno type="idhal" notation="numeric">170158</idno>
              <idno type="halauthorid" notation="string">26237-170158</idno>
              <idno type="IDREF">https://www.idref.fr/195459946</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-2288-2959</idno>
              <affiliation ref="#struct-195698"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Amélie</forename>
                <surname>de Vallée</surname>
              </persName>
              <idno type="halauthorid">499933-0</idno>
              <affiliation ref="#struct-195698"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nicolas</forename>
                <surname>Barnich</surname>
              </persName>
              <email type="md5">da5b83a9a64f3e394a10584c70e780e7</email>
              <email type="domain">uca.fr</email>
              <idno type="idhal" notation="string">nicolas-barnich</idno>
              <idno type="idhal" notation="numeric">170159</idno>
              <idno type="halauthorid" notation="string">26238-170159</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9465-7844</idno>
              <idno type="IDREF">https://www.idref.fr/183698770</idno>
              <affiliation ref="#struct-195698"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jérémy</forename>
                <surname>Denizot</surname>
              </persName>
              <email type="md5">832ec4f1b34b5910b7928b4985ef6a9a</email>
              <email type="domain">uca.fr</email>
              <idno type="idhal" notation="string">jeremy-denizot</idno>
              <idno type="idhal" notation="numeric">170025</idno>
              <idno type="halauthorid" notation="string">25951-170025</idno>
              <idno type="IDREF">https://www.idref.fr/183698592</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-0078-8996</idno>
              <affiliation ref="#struct-195698"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Claude</forename>
                <surname>Darcha</surname>
              </persName>
              <idno type="halauthorid">880129-0</idno>
              <affiliation ref="#struct-300144"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Georges</forename>
                <surname>Pignede</surname>
              </persName>
              <idno type="halauthorid">1321761-0</idno>
              <affiliation ref="#struct-530538"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Pascal</forename>
                <surname>Vandekerckove</surname>
              </persName>
              <idno type="halauthorid">1321762-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Arlette</forename>
                <surname>Darfeuille-Michaud</surname>
              </persName>
              <idno type="halauthorid">499938-0</idno>
              <affiliation ref="#struct-303623"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Jérémy</forename>
                <surname>Denizot</surname>
              </persName>
              <email type="md5">832ec4f1b34b5910b7928b4985ef6a9a</email>
              <email type="domain">uca.fr</email>
            </editor>
            <funder>Ministere de la Recherche et de la Technologie ; Institut national de la sante et de la recherche medicale UMR 1071; INRA USC 2018 ; Lesaffre International</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2018-11-20 13:30:44</date>
              <date type="whenModified">2025-03-21 15:12:20</date>
              <date type="whenReleased">2018-11-20 13:30:44</date>
              <date type="whenProduced">2015</date>
              <fs>
                <f name="inra_publicVise_local" notation="string" n="SC">
                  <string>inra_publicVise_local_SC</string>
                </f>
              </fs>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="613796">
                <persName>
                  <forename>Jérémy</forename>
                  <surname>Denizot</surname>
                </persName>
                <email type="md5">832ec4f1b34b5910b7928b4985ef6a9a</email>
                <email type="domain">uca.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-01928174</idno>
            <idno type="halUri">https://uca.hal.science/hal-01928174</idno>
            <idno type="halBibtex">sivignon:hal-01928174</idno>
            <idno type="halRefHtml">&lt;i&gt;Inflammatory Bowel Diseases&lt;/i&gt;, 2015, 21 (2), pp.276 - 286. &lt;a target="_blank" href="https://dx.doi.org/10.1097/MIB.0000000000000280"&gt;&amp;#x27E8;10.1097/MIB.0000000000000280&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Inflammatory Bowel Diseases, 2015, 21 (2), pp.276 - 286. &amp;#x27E8;10.1097/MIB.0000000000000280&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="PRES_CLERMONT">Université de Clermont</idno>
            <idno type="stamp" n="UNIV-CLERMONT1" corresp="PRES_CLERMONT">Université d'Auvergne - Clermont-Ferrand I</idno>
            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="M2ISH" corresp="PRES_CLERMONT">Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte </idno>
            <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
            <idno type="stamp" n="CHU-CLERMONTFERRAND">CHU DE CLERMONT FERRAND</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Saccharomyces cerevisiae CNCM I-3856 Prevents Colitis Induced by AIEC Bacteria in the Transgenic Mouse Model Mimicking Crohnʼs Disease</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Adeline</forename>
                    <surname>Sivignon</surname>
                  </persName>
                  <email type="md5">bc8b84f6fbc72565dfdc4885feb27115</email>
                  <email type="domain">uca.fr</email>
                  <idno type="idhal" notation="string">adeline-sivignon</idno>
                  <idno type="idhal" notation="numeric">170158</idno>
                  <idno type="halauthorid" notation="string">26237-170158</idno>
                  <idno type="IDREF">https://www.idref.fr/195459946</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-2288-2959</idno>
                  <affiliation ref="#struct-195698"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Amélie</forename>
                    <surname>de Vallée</surname>
                  </persName>
                  <idno type="halauthorid">499933-0</idno>
                  <affiliation ref="#struct-195698"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nicolas</forename>
                    <surname>Barnich</surname>
                  </persName>
                  <email type="md5">da5b83a9a64f3e394a10584c70e780e7</email>
                  <email type="domain">uca.fr</email>
                  <idno type="idhal" notation="string">nicolas-barnich</idno>
                  <idno type="idhal" notation="numeric">170159</idno>
                  <idno type="halauthorid" notation="string">26238-170159</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9465-7844</idno>
                  <idno type="IDREF">https://www.idref.fr/183698770</idno>
                  <affiliation ref="#struct-195698"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jérémy</forename>
                    <surname>Denizot</surname>
                  </persName>
                  <email type="md5">832ec4f1b34b5910b7928b4985ef6a9a</email>
                  <email type="domain">uca.fr</email>
                  <idno type="idhal" notation="string">jeremy-denizot</idno>
                  <idno type="idhal" notation="numeric">170025</idno>
                  <idno type="halauthorid" notation="string">25951-170025</idno>
                  <idno type="IDREF">https://www.idref.fr/183698592</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-0078-8996</idno>
                  <affiliation ref="#struct-195698"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Claude</forename>
                    <surname>Darcha</surname>
                  </persName>
                  <idno type="halauthorid">880129-0</idno>
                  <affiliation ref="#struct-300144"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Georges</forename>
                    <surname>Pignede</surname>
                  </persName>
                  <idno type="halauthorid">1321761-0</idno>
                  <affiliation ref="#struct-530538"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Pascal</forename>
                    <surname>Vandekerckove</surname>
                  </persName>
                  <idno type="halauthorid">1321762-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Arlette</forename>
                    <surname>Darfeuille-Michaud</surname>
                  </persName>
                  <idno type="halauthorid">499938-0</idno>
                  <affiliation ref="#struct-303623"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">14175</idno>
                <idno type="issn">1078-0998</idno>
                <idno type="eissn">1536-4844</idno>
                <title level="j">Inflammatory Bowel Diseases</title>
                <imprint>
                  <publisher>Lippincott, Williams &amp; Wilkins</publisher>
                  <biblScope unit="volume">21</biblScope>
                  <biblScope unit="issue">2</biblScope>
                  <biblScope unit="pp">276 - 286</biblScope>
                  <date type="datePub">2015</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1097/MIB.0000000000000280</idno>
              <idno type="prodinra">295509</idno>
              <idno type="wos">000348906200005</idno>
              <ref target="http://journals.lww.com/ibdjournal/pages/default.aspx" type="seeAlso"/>
              <ref type="publisher">http://journals.lww.com/ibdjournal/pages/default.aspx</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">boulardii</term>
                <term xml:lang="en">invasive Escherichia-coli</term>
                <term xml:lang="en">neoterminal ileum</term>
                <term xml:lang="en">inflammatory-bowel-disease</term>
                <term xml:lang="en">antibiotic-therapy</term>
                <term xml:lang="en">high prevalence</term>
                <term xml:lang="en">probiotic</term>
                <term xml:lang="en">yeast cell wall</term>
                <term xml:lang="en">adherent-invasive Escherichia coli</term>
                <term xml:lang="en">Crohn's disease</term>
                <term xml:lang="fr">Saccharomyces cerevisiae</term>
                <term xml:lang="fr">microbiota</term>
                <term xml:lang="fr">T84 cell</term>
                <term xml:lang="fr">ileal mucosa</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.aen">Life Sciences [q-bio]/Food and Nutrition</classCode>
              <classCode scheme="halDomain" n="sdv.bc.ic">Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]</classCode>
              <classCode scheme="halDomain" n="sdv.imm.ii">Life Sciences [q-bio]/Immunology/Innate immunity</classCode>
              <classCode scheme="halDomain" n="sdv.mp.bac">Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bm">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.gtp">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Background:Adherent-invasive Escherichia coli (AIEC), which colonize the ileal mucosa of patients with Crohn's disease (CD), are able to adhere to and invade intestinal epithelial cells. Overexpression of the glycoprotein CEACAM6 on host cells favors AIEC attachment and inflammation. We investigated the ability of Saccharomyces cerevisiae CNCM I-3856 to inhibit AIEC adhesion and to reduce colitis.Methods:Adhesion experiments were performed on T84 cells and on enterocytes from patients with CD with AIEC LF82 in the presence of S. cerevisiae. Colonization and symptoms of colitis were assessed in LF82-infected transgenic CEABAC10 mice treated with live S. cerevisiae or S. cerevisiae derivatives. Proinflammatory cytokines were quantified by enzyme linked immunosorbent assay. Intestinal permeability was assessed by measuring the 4 kDa dextran-FITC flux in the serum.Results:S. cerevisiae strongly inhibited LF82 adhesion to T84 cells and to the brush border of CD enterocytes. Yeasts decreased LF82 colonization and colitis in CEABAC10 mice and restored barrier function through prevention of the LF82-induced expression of pore-forming tight junction claudin-2 at the plasma membrane of intestinal epithelial cells. These effects were accompanied by a decrease in proinflammatory cytokines IL-6, IL-1, and KC release by the gut mucosa. Yeast derivatives exerted similar effects on LF82 colonization and colitis demonstrating that yeast viability was not essential to exert beneficial effects.Conclusions:S. cerevisiae yeasts reduce colitis induced by AIEC bacteria in CEACAM6-expressing mice. Such a probiotic strategy could be envisaged in a subgroup of patients with CD abnormally expressing CEACAM6 at the ileal mucosa and therefore susceptible to being colonized by AIEC bacteria.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-195698" status="OLD">
          <idno type="IdRef">202422275</idno>
          <idno type="RNSR">201220155K</idno>
          <orgName>Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte</orgName>
          <orgName type="acronym">M2iSH</orgName>
          <date type="start">2005-01-01</date>
          <date type="end">2016-12-31</date>
          <desc>
            <address>
              <addrLine>UFR de Médecine et de Pharmacie, 28 Place Henri Dunant, BP 38, 63001 Clermont-Ferrand Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.u-clermont1.fr/m2ish.html</ref>
          </desc>
          <listRelation>
            <relation name="UR2018" active="#struct-92114" type="direct"/>
            <relation name="UMR1071" active="#struct-300267" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-300144" status="VALID">
          <idno type="IdRef">026606836</idno>
          <idno type="ISNI">0000000406394151</idno>
          <idno type="ROR">https://ror.org/02tcf7a68</idno>
          <orgName>CHU Clermont-Ferrand</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.chu-clermontferrand.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-530538" status="INCOMING">
          <orgName>Division R&amp;D</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-530537" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-92114" status="OLD">
          <idno type="ROR">https://ror.org/01x3gbx83</idno>
          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300267" status="OLD">
          <orgName>Université d'Auvergne - Clermont-Ferrand I</orgName>
          <orgName type="acronym">UdA</orgName>
          <date type="end">2016-12-31</date>
          <desc>
            <address>
              <addrLine>49, Boulevard François-Mitterrand / CS 60032 / 63001 Clermont-Ferrand Cedex 1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.u-clermont1.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-530537" status="INCOMING">
          <orgName>Lesaffre International</orgName>
          <desc>
            <address>
              <addrLine>Marcq-en-Baroeul</addrLine>
              <country key="FR"/>
            </address>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>