%0 Journal Article %T Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli %+ Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF) %+ Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH) %+ Institut Fédératif de Recherche 142 %+ Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN) %+ NanoBioInterfaces - IEMN (NBI - IEMN) %+ Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate %+ Laboratoire de Synthèse Organique (Hétérochimie organique, organoéléments et matériaux) (LSOHOOM) %+ Universidad de Cantabria [Santander] %+ Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) %+ Laboratoire de Chimie Organique et Macromoleculaire (UMR CNRS 8009) %+ Danylo Halytskiy Lviv National Medical University %A Dumych, Tetiana %A Yamakawa, Nao %A Sivignon, Adeline %A Garénaux, Estelle %A Robakiewicz, Stefania %A Coddeville, Bernadette %A Bongiovanni, Antonino %A Bray, Fabrice %A Barnich, Nicolas %A Szunerits, Sabine %A Slomianny, Christian %A Herrmann, Martin %A Gouin, Sébastien %A Lutsyk, Alexander %A Munoz, Luis %A Lafont, Frank %A Rolando, Christian %A Bilyy, Rostyslav %A Bouckaert, Julie %Z Ministry of Healthcare of Ukraine 0116U000759; WUBMRC; Horizon RISE project 690836; French Agence Nationale de la Recherche ANR-12-BSV5-0016-01; ANR-15-CE15-0017-03; Ardea Biosciences, Inc.; DFG KFO257; VolksWagen Stiftung 90361 %< avec comité de lecture %@ 1664-302X %J Frontiers in Microbiology %I Frontiers Media %V 9 %8 2018-04-18 %D 2018 %R 10.3389/fmicb.2018.00742 %M 29720971 %K oligomannose glycans %K apoptotic cell-derived membranous vesicles %K adherent-invasive E coli %K CEACAM6 %K Crohn's disease %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X A novel mechanism is revealed by which clinical isolates of adherent-invasive Escherichia coli (AIEC) penetrate into the epithelial cell layer, replicate, and establish biofilms in Crohn's disease. AIEC uses the FimH fimbrial adhesin to bind to oligomannose glycans on the surface of host cells. Oligomannose glycans exposed on early apoptotic cells are the preferred binding targets of AIEC, so apoptotic cells serve as potential entry points for bacteria into the epithelial cell layer. Thereafter, the bacteria propagate laterally in the epithelial intercellular spaces. We demonstrate oligomannosylation at two distinct sites of a glycoprotein receptor for AIEC, carcinoembryonic antigen related cell adhesion molecule 6 (CEACAM6 or CD66c), on human intestinal epithelia. After bacterial binding, FimH interacts with CEACAM6, which then clusters. The presence of the highest-affinity epitope for FimH, oligomannose-5, on CEACAM6 is demonstrated using LC-MS/MS. As mannose-dependent infections are abundant, this mechanism might also be used by other adherent-invasive pathogens. %G English %2 https://uca.hal.science/hal-01796957/document %2 https://uca.hal.science/hal-01796957/file/fmicb-09-00742.pdf %L hal-01796957 %U https://uca.hal.science/hal-01796957 %~ INSERM %~ UNIV-NANTES %~ RIIP %~ PRES_CLERMONT %~ CNRS %~ UNIV-VALENCIENNES %~ ENSC-LILLE %~ INRA %~ IEMN %~ ACL-SVSAE %~ M2ISH %~ AGREENIUM %~ UNIV-LILLE %~ INRAE %~ CIIL %~ TEST-HALCNRS %~ CEISAM %~ NANTES-UNIVERSITE %~ UNIV-NANTES-AV2022 %~ TEST3-HALCNRS