Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara - Université Clermont Auvergne Access content directly
Journal Articles Carbohydrate Polymers Year : 2017

Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara

Abstract

A water soluble polysaccharide (WSP) was extracted and purified from Astragalus gombo seeds (Fabaceae) harvested in Septentrional Sahara (Ouargla, Algeria) with a yield of 6.8% (w/w of the dry seed ground). It was characterized by gas chromatography coupled to the mass spectrometry (GC–MS), size exclusion chromatography with Multi-Angle Light Scattering analysis (SEC-MALLS), high–resolution 1H and 13C NMR, and rheological measurements. The structural characterization indicated that this WSP fraction is a galactomannan with a mannose/galactose ratio of 1.7 formed by a backbone of β-(1,4)-d-mannopyranosyl residues (63%) substituted at O-6 position by a single α-galactopyranose residue (37%). SEC-MALLS analysis revealed that this galactomannan has an average molecular mass (Mw) of 1.1 × 106 g/mol, an intrinsic viscosity of 860 mL/g and, a random coil conformation structure. Rheological analysis in semi diluted regimes shown pseudo-plastic and viscoelastic behaviour.
No file

Dates and versions

hal-01658277 , version 1 (07-12-2017)

Identifiers

Cite

Toufik Chouana, Guillaume Pierre, Christophe Vial, Christine Gardarin, Anne Wadouachi, et al.. Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara. Carbohydrate Polymers, 2017, 175, pp.387 - 394. ⟨10.1016/j.carbpol.2017.08.003⟩. ⟨hal-01658277⟩
131 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More