Twelve polymorphic expressed sequence tags-derived markers for Plasmopara halstedii, the causal agent of sunflower downy mildew - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Molecular Ecology Notes Année : 2007

Twelve polymorphic expressed sequence tags-derived markers for Plasmopara halstedii, the causal agent of sunflower downy mildew

Résumé

Twelve expressed sequence tags-derived markers were isolated from Plasmopara halstedii (Oomycetes), the causal agent of sunflower downy mildew. A total of 25 single nucleotide polymorphisms and five indels were detected by single-strand conformation polymorphism analysis and developed for high-throughput genotyping of 32 isolates. There was a high level of genetic diversity (HE = 0.484). Observed heterozygosity ranged from 0 to 0.143 indicating that P. halstedii is probably a selfing species. These markers were also useful in detecting significant genetic variations among French populations (FST = 0.193) and between French and Russian populations (FST = 0.23). Cross-amplification tests on three closely related species indicated that no loci amplified in other Oomycete species.

Dates et versions

hal-00964293 , version 1 (24-03-2014)

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Citer

Xavier X. Giresse, Denis D. Tourvieille de Labrouhe, Sylvie Richart-Cervera Richard Cervera, François F. Delmotte. Twelve polymorphic expressed sequence tags-derived markers for Plasmopara halstedii, the causal agent of sunflower downy mildew. Molecular Ecology Notes, 2007, 7, pp.1363-1365. ⟨10.1111/j.1471-8286.2007.01887.x⟩. ⟨hal-00964293⟩
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