Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas-chromatography mass spectrometry. Application to polytraumatized patients - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences Année : 2008

Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas-chromatography mass spectrometry. Application to polytraumatized patients

Résumé

Isoprostanes are a family of prostaglandin isomers produced fromoxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F2-isoprostanes (F2-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since „interferences peaks‰ at m/z 573 co-elute with d4-15-F2t-IsoP preferentially used, we propose a new GCˆNICI-MS approach to quantify urinary F2-IsoPs by using 4(RS)-F4t-neuroprostane as the internal standard. This method was applied to quantify urinary F2-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p < 0.0001) in urinary F2-IsoPs in polytraumatized patients compared with healthy volunteers (4.73±2.75 ng/mg vs. 0.811±0.359 ng/mg creatinine).

Domaines

Chimie organique

Dates et versions

hal-00319300 , version 1 (08-09-2008)

Identifiants

Citer

Emilie Mas, Françoise Michel, Alexandre Guy, Valerie Bultel-Poncé, Yoan Falquet, et al.. Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas-chromatography mass spectrometry. Application to polytraumatized patients. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2008, 872, pp.133-140. ⟨10.1016/j.jchromb.2008.07.037⟩. ⟨hal-00319300⟩
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