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Article Dans Une Revue European Journal of Nutrition Année : 2018

Metabolic adaptations to HFHS overfeeding: how whole body and tissues postprandial metabolic flexibility adapt in Yucatan mini-pigs

Didier Remond
Annick Bernalier
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Mathieu Rambeau
Estelle Pujos-Guillot
Blandine Comte
Dominique Dardevet

Résumé

Purpose In the present study, we aimed to metabolically characterize the postprandial adaptations of the major tissues involved in energy, lipids and amino acids metabolisms in mini-pigs. Method Mini-pigs were fed on high-fat–high-sucrose (HFHS) diet for 2 months and several tissues explored for metabolic analyses. Further, the urine metabolome was followed over the time to picture the metabolic adaptations occurring at the whole body level following overfeeding. Results After 2 months of HFHS consumption, mini-pigs displayed an obese phenotype characterized by high circulating insulin, triglycerides and cholesterol levels. At the tissue level, a general (muscle, adipose tissue, intestine) reduction in the capacity to phosphorylate glucose was observed. This was also supported by the enhanced hepatic gluconeogenesis potential, despite the concomitant normoglycaemia, suggesting that the high circulating insulin levels would be enough to maintain glucose homoeostasis. The HFHS feeding also resulted in a reduced capacity of two other pathways: the de novo lipogenesis, and the ranched-chain amino acids transamination. Finally, the follow-up of the urine metabolome over the time allowed determining breaking points in the metabolic trajectory of the animals. Conclusions Several features confirmed the pertinence of the animal model, including increased body weight, adi- posity and porcine obesity index. At the metabolic level,we observed a perturbed glucose and amino acid metabolism, known to be related to the onset of the obesity. The urine metabolome analyses revealed several metabolic pathways potentially involved in the obesity onset, including TCA (citrate, pantothenic acid), amino acids catabolism (cysteine, threonine, leucine).
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Dates et versions

hal-01525343 , version 1 (19-05-2017)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Citer

Sergio Polakof, Didier Remond, Annick Bernalier, Mathieu Rambeau, Estelle Pujos-Guillot, et al.. Metabolic adaptations to HFHS overfeeding: how whole body and tissues postprandial metabolic flexibility adapt in Yucatan mini-pigs. European Journal of Nutrition, 2018, 57 (1), pp.119-135. ⟨10.1007/s00394-016-1302-1⟩. ⟨hal-01525343⟩
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